How Plant Extracts are Prepared ?

The collected plant material (plant parts: stems, leaves, flowers, roots, bark etc.) is dried in a ventilated oven at 45 oC for 24 H, and subsequently milled to a fine powder by means of an IKA Universal Mixer M20 (or other type). An amount of 20.0 g of the dried plant powder is weighed in an Erlenmeyer of 100 ml to which 70 ml of hexane (purity grade 99 %) is added (the plant sample has to be submerged with solvent) for pre-extraction. The Erlenmeyer is placed in a sonicator-bath (Branson 8210 or some other type) and sonicated at a temperature 40 oC during 30 minutes. The mixture is filtered using paper filter, followed by washing the Erlenmeyer with 20 ml of hexane and then with 50 ml of hexane. The filtrate is poured in a round-bottomed flask and the solvent is concentrated in vacuo (at about 11 mm Hg) up to 5-10 ml by means of rotavapor, utilizing a water bath at 40 oC. This residue is brought in a 30-ml vessel to let the solvent evaporate. The open vessel is left overnight in a well-ventilated hood in order to evaporate the last traces of the solvent in the hexane pre-extract.
 

The solids, collected on the filter, are broken up and dried in the air overnight in the hood. The dried material is extracted in the same way with methanol-water (90:10). The dried material from the filters placed in an Erlenmeyer of 100 ml to which 70 ml of 90 % methanol is added. The mixture is sonicated as above at 40C during 30 minutes, after which it is filtered, followed by washing the Erlenmeyer with 20 ml of 90 % methanol. The filtrate is poured in a round-bottomed flask and the solvent is evaporated in vacuo completely. The dry 90% methanol extract is dissolved in as little as possible 100 % methanol by using the sonicator-bath and poured in a 30-ml vessel to let it evaporate overnight in the hood.

Xi’an FineSky Technological Co.,Ltd. is a professional plant extract manufacturer in China,FineSky shows strong production ability and could supply many kinds of plant extract.Our products have been exported to many countries in the world.We have excellent R&D Capabilities,Strict quality control system and experienced team.We believe we have the capability to supply premier products .FineSky will build the human nutrition,health ,beauty and high-quality life with our clients.We will supply continoually exceptional scientific, regulatory, technical and marketing support .

We can supply plant extract,including hops extract,artichoke extract,green tea extract,milk thistle extract,epimedium extract,St.John's wort extract,grape seeds extract,soybean extract,yohimbe bark extract,common cnidium extract,matrine extract,olive leaf extract,goji extract,goji dried fruit,green coffee bean extract,coenzyme Q10,pomegranate extract,kudzu extract,tribulus terrestris extract,hawthorn extract,angelica extract,dandelion extract,garlic extract,ginseng extract,echinacea purpurea extract,white willow extract,red clover extract,polygonum cuspidatum extract,pine bark extract,raspberry extract,etc.